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Making cRNA for microinjection and expression of fluorescently tagged proteins for live-cell imaging in oocytes.
Methods in molecular biology (Clifton, N.J.) 2013Sizes of these terms reflect their relevance to your search.
Fluorescently tagged proteins have become a crucial weapon in the armory of a successful cell biology laboratory. This chapter describes how to produce cRNA coding for a fluorescently tagged protein of choice, such that it is suitable for microinjection and subsequent expression studies in live oocytes.
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Mark Levasseur. Making cRNA for microinjection and expression of fluorescently tagged proteins for live-cell imaging in oocytes. Methods in molecular biology (Clifton, N.J.). 2013;957:121-34
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PMID: 23138948
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