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Fluorescently tagged proteins have become a crucial weapon in the armory of a successful cell biology laboratory. This chapter describes how to produce cRNA coding for a fluorescently tagged protein of choice, such that it is suitable for microinjection and subsequent expression studies in live oocytes.


Mark Levasseur. Making cRNA for microinjection and expression of fluorescently tagged proteins for live-cell imaging in oocytes. Methods in molecular biology (Clifton, N.J.). 2013;957:121-34

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PMID: 23138948

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