Preparation of an adipogenic hydrogel from subcutaneous adipose tissue.

Christopher J Poon, Maria V Pereira E Cotta, Shiba Sinha, Jason A Palmer, Alan A Woods, Wayne A Morrison, Keren M Abberton

O'Brien Institute, Fitzroy, Victoria, Australia. christopher.poon@alumni.unimelb.edu.au

Acta biomaterialia 2013 Mar

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The ability to generate controlled amounts of adipose tissue would greatly ease the burden on hospitals for reconstructive surgery. We have previously shown that a tissue engineering chamber containing a vascular pedicle was capable of forming new fat; however, further refinements are required to enhance fat formation. The development and maintenance of engineered adipose tissue requires a suitable source of growth factors and a suitable scaffold. A hydrogel derived from adipose tissue may fulfil this need. Subcutaneous fat was processed into a thermosensitive hydrogel we refer to as adipose-derived matrix (ADM). Protein analysis revealed high levels of basement membrane proteins, collagens and detectable levels of growth factors. Adipose-derived stem cells exposed to this hydrogel differentiated into adipocytes with >90% efficiency and in vivo testing in rats showed significant signs of adipogenesis after 8 weeks. ADM's adipogenic properties combined with its simple gelation, relatively long shelf life and its tolerance to multiple freeze-thaw cycles, makes it a promising candidate for adipose engineering applications. Copyright © 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Citation

Christopher J Poon, Maria V Pereira E Cotta, Shiba Sinha, Jason A Palmer, Alan A Woods, Wayne A Morrison, Keren M Abberton. Preparation of an adipogenic hydrogel from subcutaneous adipose tissue. Acta biomaterialia. 2013 Mar;9(3):5609-20