Impulse-dependent extracellular resting dopamine concentration in rat striatum in vivo.

Pan-Li Zuo, Wei Yao, Liang Sun, Shu-Ting Kuo, Qing Li, Shi-Rong Wang, Hai-Qiang Dou, Hua-Dong Xu, Claire Xi Zhang, Xin-Jiang Kang, Zhuan Zhou, Bo Zhang

State Key Laboratory of Biomembrane and Membrane Biotechnology and the Center for Life Sciences, Institute of Molecular Medicine, Peking University, Beijing 100871, China.

Neurochemistry international 2013 Jan

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The ambient resting dopamine (DA) concentration in brain regulates cognition and motivation. Despite its importance, resting DA level in vivo remains elusive. Here, by high-frequency stimulation of the medial forebrain bundle and immediately following the stimulus-induced DA overflow, we recorded a DA "undershoot" which is a temporal reduction of DA concentration to a level below the baseline. Based on the DA undershoot, we predicted a resting DA concentration of ∼73nM in rat striatum in vivo. Simulation studies suggested that removing basal DA by DAT during the post-stimulation inhibition of tonic DA release caused the DA undershoot, and the resting concentration of DA modulated the kinetics of the evoked DA transient. The DA undershoot was eliminated by either blocking D2 receptors with haloperidol or blocking the DA transporter (DAT) with cocaine. Therefore, the impulse-dependent resting DA concentration is in the tens of nanomolar range and is modulated by the presynaptic D2 receptors and the DAT in vivo. Crown Copyright © 2012. Published by Elsevier Ltd. All rights reserved.

Citation

Pan-Li Zuo, Wei Yao, Liang Sun, Shu-Ting Kuo, Qing Li, Shi-Rong Wang, Hai-Qiang Dou, Hua-Dong Xu, Claire Xi Zhang, Xin-Jiang Kang, Zhuan Zhou, Bo Zhang. Impulse-dependent extracellular resting dopamine concentration in rat striatum in vivo. Neurochemistry international. 2013 Jan;62(1):50-7