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We reported an efficient strategy based on a strand displacement amplification for serum miRNA detection. In such a system, a multiplexed, sensitive and quick detection of miRNAs could be achieved through a combination of fluorescence labeled probes, a common primer and a polymerase. This could be potentially used in the clinical field to achieve early disease diagnosis and prognosis.


Tian Tian, Heng Xiao, Xiaolian Zhang, Shuang Peng, Xiaoe Zhang, Shan Guo, Shaoru Wang, Songmei Liu, Xin Zhou, Craig Meyers, Xiang Zhou. Simultaneously sensitive detection of multiple miRNAs based on a strand displacement amplification. Chemical communications (Cambridge, England). 2013 Jan 4;49(1):75-7

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PMID: 23159915

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