Transient receptor potential N (TRPN1) from Xenopus interacts with the penta-EF-hand protein peflin.
Dominik Wiemuth, Lena van de Sandt, Rudolf Herr, Stefan Gründer
Department of Physiology, RWTH Aachen University, 52074 Aachen, Germany.
FEBS letters 2012 Dec 14TRPN1 is a candidate mechanotransduction channel in Drosophila and Caenorhabditis elegans, also present in hair cells of lower vertebrates. At its N-terminal cytoplasmic tail it contains 28 ankyrin repeats. We performed a yeast two-hybrid screen with the N-terminal ankyrin repeats of Xenopus TRPN1 as bait and identified the Penta-EF-hand protein peflin as a putative interaction partner. We confirmed this interaction by GST pulldown assays and by co-localization in an epithelial cell model. Collectively, our study identifies peflin as an interaction partner of TRPN1 in vitro. Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Citation
Dominik Wiemuth, Lena van de Sandt, Rudolf Herr, Stefan Gründer. Transient receptor potential N (TRPN1) from Xenopus interacts with the penta-EF-hand protein peflin. FEBS letters. 2012 Dec 14;586(24):4276-81
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PMID: 23159937
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