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Keeping a broad (RNA) perspective: conventional biochemical detection systems only have a 100-fold linear response range. The range of potassium concentrations detected by an RNA G-quadruplex sequence can be broadened by intentionally populating multiple intermediate folding states. The folding of the RNA G-quadruplexes was monitored by both circular dichroism and intrinsic fluorescence spectroscopy. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Chun Kit Kwok, Madeline E Sherlock, Philip C Bevilacqua. Decrease in RNA folding cooperativity by deliberate population of intermediates in RNA G-quadruplexes. Angewandte Chemie (International ed. in English). 2013 Jan 7;52(2):683-6

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PMID: 23166020

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