Impact of peptide micropatterning on endothelial cell actin remodeling for cell alignment under shear stress.

Céline Chollet, Reine Bareille, Murielle Rémy, Alain Guignandon, Laurence Bordenave, Gaetan Laroche, Marie-Christine Durrieu

Bioingénierie Tissulaire, Université Bordeaux, U1026, F-33000 Bordeaux, France.

Macromolecular bioscience 2012 Dec

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HSVEC behavior under physiological shear stress in vitro is investigated on PET surfaces micropatterned with both RGDS and WQPPRARI peptides. This technique allows (i) creating geometries on surface to guide cell orientation under shear stress and (ii) controlling surface chemical composition in order to modulate cell behavior. Under shear stress, endothelial cells adhere on patterned PET surfaces and present a more rapid orientation in flow direction in comparison to cells cultured on homogeneous surfaces. Micropatterned surfaces presenting a large surface area ratio of RGDS/WQPPRARI peptides induce fibrillar adhesion, while surfaces presenting an equal RGDS/WQPPRARI peptides surface area ratio preferentially induce focal adhesion. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citation

Céline Chollet, Reine Bareille, Murielle Rémy, Alain Guignandon, Laurence Bordenave, Gaetan Laroche, Marie-Christine Durrieu. Impact of peptide micropatterning on endothelial cell actin remodeling for cell alignment under shear stress. Macromolecular bioscience. 2012 Dec;12(12):1648-59