Borate enhances the production of lactulose from lactose by cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus.

Yeong-Su Kim, Jung-Eun Kim, Deok-Kun Oh

Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.

Bioresource technology 2013 Jan

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Cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus was used in the presence of borate to increase the production of lactulose from lactose. Maximum production of lactulose occurred using a 1:1M ratio of borate-lactose. Under this condition, the enzyme produced 614 g l(-1) lactulose from 700 g l(-1) lactose after incubation at pH 7.5 and 80 °C for 3h, with a conversion yield of 88% and a productivity of 205 g l(-1) h(-1). The yield and productivity of lactulose production obtained in the present study are among the highest achieved through chemical or biological synthesis. Crown Copyright © 2012. Published by Elsevier Ltd. All rights reserved.

Citation

Yeong-Su Kim, Jung-Eun Kim, Deok-Kun Oh. Borate enhances the production of lactulose from lactose by cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus. Bioresource technology. 2013 Jan;128:809-12