Recruitment and rearrangement of three different genetic determinants into a conjugative plasmid increase copper resistance in Pseudomonas syringae.

José A Gutiérrez-Barranquero, Antonio de Vicente, Víctor J Carrión, George W Sundin, Francisco M Cazorla

Applied and environmental microbiology 2013 Feb

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We describe the genetic organization of a copper-resistant plasmid containing copG and cusCBA genes in the plant pathogen Pseudomonas syringae. Chromosomal variants of czcCBA and a plasmid variant of cusCBA were present in different P. syringae pathovar strains. Transformation of the copper-sensitive Pseudomonas syringae pv. syringae FF5 strain with copG or cusCBA conferred copper resistance, and quantitative real-time PCR (qRT-PCR) experiments confirmed their induction by copper.

Citation

José A Gutiérrez-Barranquero, Antonio de Vicente, Víctor J Carrión, George W Sundin, Francisco M Cazorla. Recruitment and rearrangement of three different genetic determinants into a conjugative plasmid increase copper resistance in Pseudomonas syringae. Applied and environmental microbiology. 2013 Feb;79(3):1028-33