Determination of carboxyatractyloside and atractyloside in Xanthii Fructus by HPLC].

To determine carboxyatractyloside and atractyloside in Xanthii Fructus by HPLC. By HPLC, Agilent ZORBAX SB-phenyl (4.6 mm x 250 mm, 5 microm) column was adopted, with acetonitrile-0.01 mol x L(-1) NaH2PO4 (pH 6) as the mobile phase for gradient elution at the flow rate of 1.0 mL x min(-1). The detection wavelength was 203 nm, and the temperature was set at 35 degrees C. Carboxyatractyloside showed a good linearity within the range of 0.0972-1.944 microg and atractyloside showed a good linearity within the range of 0.1030-2.060 microg. The recovery rate of carboxyatractyloside was 100. 3% and that of atractyloside was 102.5%. The RSD were 0.67% and 1.4% (n=6). This method is so simple, practical and highly repeatable that is can be used for quality control of Xanthii Fructus.

Citation

Rui Duo, Yan Chen, Yuhong Liu, Zhifang Huang, Yunhua Liu, Jinhai Yi. Determination of carboxyatractyloside and atractyloside in Xanthii Fructus by HPLC]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 2012 Aug;37(15):2313-6

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PMID: 23189741

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