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The research introduced a novel method for gene replacement in intact Bacillus subtilis by employing full-length single-stranded (ss) DNA constructs and electro-transformation. 5' phosphorothioated lagging-strand targeting ssDNA construct was demonstrated to be highly recombinogenic, and the utility of the system was illustrated by introducing a heterologous lipase YlLip2 into amyE locus of B. subtilis through our method. Copyright © 2012 Elsevier B.V. All rights reserved.


Sai Wen, Jianguo Yang, Tianwei Tan. Full-length single-stranded PCR product mediated chromosomal integration in intact Bacillus subtilis. Journal of microbiological methods. 2013 Mar;92(3):273-7

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PMID: 23201169

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