Effect of branching density on avidity of hyperbranched glycomimetics for mannose binding lectin.

Department of Bioengineering, University of California-Los Angeles, 410 Westwood Plaza, Los Angeles, CA 90095-1600, United States.

Biomacromolecules 2013 Feb 11

filter terms:

Hyperbranched glycopolymers containing mannose units in the branch point were synthesized through the copolymerization of a mannose inimer and mannose acrylate via atom transfer radical polymerization (ATRP). Incorporating a saccharide residue at the branch point results in a closer analogue to natural branched polysaccharides. Gel permeation chromatography characterization of the polymers qualitatively indicates branching in samples from polymerizations utilizing the mannose inimer. Deprotection of the acetate protecting groups from the hyperbranched mannose polymers yields water-soluble polymers that interact with mannose binding lectin (MBL), a key protein of the innate immunity complement system. MBL interaction increases with increasing polymer mole cular weight and increasing branching density. Notably, incorporating mannose into the branching repeat unit also increases the interaction of the glycopolymers with MBL compared with glycopolymers with the same branching density but with no mannose at the branch point.

Citation

Kenneth Lin, Andrea M Kasko. Effect of branching density on avidity of hyperbranched glycomimetics for mannose binding lectin. Biomacromolecules. 2013 Feb 11;14(2):350-7