Proteomic analysis of retinopathy-related plasma biomarkers in diabetic patients.

Chieh-Hsiang Lu, Szu-Ting Lin, Hsiu-Chuan Chou, Ying-Ray Lee, Hong-Lin Chan

Division of Endocrinology and Metabolism, Department of Internal Medicine, Chiayi Christian Hospital, Chiayi, Taiwan.

Archives of biochemistry and biophysics 2013 Jan 15

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Diabetic retinopathy occurs in approximately 25% of patients with type 1 or type 2 diabetes; the disease can cause poor vision and even blindness because high glucose levels weaken retinal capillaries, causing leakage of blood into surrounding areas. We adopted a proteomics-based approach using 2D-DIGE and MALDI-TOF/TOF MS to compare the differential plasma proteome between diabetic retinopathy with significant retinopathy occurrence within 5years after diagnosis of diabetes, and diabetic non-retinopathy without diagnosed retinopathy for more than 10years after diagnosis of diabetes. We identified 77 plasma proteins, which represent 28 unique gene products. These proteins mainly have inflammatory response and coagulation roles. Our approach identified several potential diabetic retinopathy biomarkers including afamin and the protein arginine N-methyltransferase 5, which may be associated with the progression and development of diabetes. In conclusion, we report a comprehensive patient-based plasma proteomic approach to the identification of potential plasma biomarkers for diabetic retinopathy screening and detection. Copyright © 2012 Elsevier Inc. All rights reserved.

Citation

Chieh-Hsiang Lu, Szu-Ting Lin, Hsiu-Chuan Chou, Ying-Ray Lee, Hong-Lin Chan. Proteomic analysis of retinopathy-related plasma biomarkers in diabetic patients. Archives of biochemistry and biophysics. 2013 Jan 15;529(2):146-56