Efficient synthesis of (+)-cis-12-oxo-phytodienoic acid by an in vitro enzymatic reaction.

Akiyuki Kajiwara, Tatsuya Abe, Takahiro Hashimoto, Hideyuki Matsuura, Kosaku Takahashi

Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan.

Bioscience, biotechnology, and biochemistry 2012

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12-Oxo-phytodienoic acid (OPDA) is an important metabolite on the octadecanoid pathway of plants. This study shows an efficient in vitro synthesis of (+)-cis-OPDA by using a flaxseed extract and an allene oxide cyclase. The OPDA yield of the reaction in this study was almost 7-fold higher than that in the conventional reaction with the flaxseed extract.

Citation

Akiyuki Kajiwara, Tatsuya Abe, Takahiro Hashimoto, Hideyuki Matsuura, Kosaku Takahashi. Efficient synthesis of (+)-cis-12-oxo-phytodienoic acid by an in vitro enzymatic reaction. Bioscience, biotechnology, and biochemistry. 2012;76(12):2325-8