Considerations on regulatory sequences of the distal promoter region of the HLA-G gene.

José Samuel da Silva, Renata Slowik, Maria da Graça Bicalho

Laboratório de Imunogenética e Histocompatibilidade-LIGH, Departamento de Genética, Centro Politécnico, Universidade Federal do Paraná-UFPR, Av. Coronel Francisco H. dos Santos, Jardim das Américas. Curitiba, Paraná, CEP 81530990, Caixa Postal 19071, Brazil. samuel-bio@hotmail.com

Human immunology 2013 Apr

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Gene expression in eukaryotic cells is accomplished via association of transcription factors, some of which directly bind to DNA regulatory sequences. HLA-G codes for an immunoregulatory protein with tissue-specific expression, its unique promoter regulatory region is responsible for this feature. The aim of the present study was to explore motif composition as well as identify haplotypes in the HLA-G 5' distal promoter region. The sample was composed by 176 euro-descendents individuals genotyped by Sequence Based Typing of HLA-G distal promoter, encompassing 16 SNPs. Haplotypes were inferred by the expectation maximization algorithm. Only haplotypes with frequency higher than 1% were aligned to check for similarities and differences and thirteen haplotypes remained. For a better understanding of the nucleotide diversity of the analyzed region our approach was to split the whole sequence into two regions. Two contrasting haplotype groups were found in both regions, allowing us to suggest the existence of different transcription factors capable of binding cis elements while the intra-group variations suggest the intensity modulation of binding with regulatory factors. Copyright © 2012 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.

Citation

José Samuel da Silva, Renata Slowik, Maria da Graça Bicalho. Considerations on regulatory sequences of the distal promoter region of the HLA-G gene. Human immunology. 2013 Apr;74(4):473-7