Optimizing the enzyme loading and incubation time in enzymatic hydrolysis of lignocellulosic substrates.

Roger H Newman, Alankar A Vaidya, M Imroz Sohel, Michael W Jack

Scion, Private Bag 3020, Rotorua Mail Centre, Rotorua 3046, New Zealand. Roger.Newman@scionresearch.com

Bioresource technology 2013 Feb

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A mathematical model for costing enzymatic hydrolysis of lignocellulosics is presented. This model is based on three variable parameters describing substrate characteristics and three unit costs for substrate, enzymes and incubation. The model is used to minimize the cost of fermentable sugars, as intermediate products on the route to ethanol or other biorefinery products, by calculating optimized values of enzyme loading and incubation time. This approach allows comparisons between substrates, with processing conditions optimized independently for each substrate. Steam-exploded pine wood was hydrolyzed in order to test the theoretical relationship between sugar yield and processing conditions. Copyright © 2012 Elsevier Ltd. All rights reserved.

Citation

Roger H Newman, Alankar A Vaidya, M Imroz Sohel, Michael W Jack. Optimizing the enzyme loading and incubation time in enzymatic hydrolysis of lignocellulosic substrates. Bioresource technology. 2013 Feb;129:33-8