Visualizing cell structure and function with point-localization superresolution imaging.

Prabuddha Sengupta, Schuyler Van Engelenburg, Jennifer Lippincott-Schwartz

Developmental cell 2012 Dec 11

filter terms:

Fundamental to the success of cell and developmental biology is the ability to tease apart molecular organization in cells and tissues by localizing specific proteins with respect to one another in a native cellular context. However, many key cellular structures (from mitochondrial cristae to nuclear pores) lie below the diffraction limit of visible light, precluding analysis of their organization by conventional approaches. Point-localization superresolution microscopy techniques, such as PALM and STORM, are poised to resolve, with unprecedented clarity, the organizational principles of macromolecular complexes within cells, thus leading to deeper insights into cellular function in both health and disease. Copyright © 2012 Elsevier Inc. All rights reserved.

Citation

Prabuddha Sengupta, Schuyler Van Engelenburg, Jennifer Lippincott-Schwartz. Visualizing cell structure and function with point-localization superresolution imaging. Developmental cell. 2012 Dec 11;23(6):1092-102