Effect of olprinone on liver microstructure in rat partial liver transplantation.

Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

The Journal of surgical research 2013 Jul

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Donor safety is a major concern in living-donor liver transplantation. However, partial grafts do not meet the functional demands of recipients and lead to small-for-size syndrome (SFSS). In a previous study, we showed that olprinone (OLP), a selective phosphodiesterase ІІІ inhibitor, up-regulates endothelial nitric oxide synthase level in the liver and attenuates shear stress, sinusoidal endothelial cell injury, and hepatocyte apoptosis after excessive liver resection in a rat model. We aimed to examine whether OLP treatment has beneficial effects on SFSS in a rat model of partial liver transplantation (PLT). We performed experiments in a rat model of 30% PLT. In the OLP group, we inserted an osmotic pump with OLP into the peritoneal cavity 48 h before liver graft sampling. Recipient rats were not treated with OLP. We examined the liver microstructure by electron microscopy and biochemical examination, and determined the 7-d survival of recipients. In the OLP group 1 h after PLT, the sinusoidal endothelial cells of the liver were well preserved and we observed few vacuolar structures in hepatocytes. The total serum bilirubin level 1 wk after PLT tended to be lower in the OLP group than in the controls, and the liver microstructures were also well preserved in the OLP group. The probability of survival in the OLP group (100%; 14 of 14 rats) was significantly higher than that in the control group (75%; 15 of 20 rats). Olprinone treatment was demonstrated to have therapeutic potential to overcome SFSS. Copyright © 2013 Elsevier Inc. All rights reserved.

Citation

Kenya Yamanaka, Etsuro Hatano, Khota Iguchi, Gen Yamamoto, Motohiko Sato, Kan Toriguchi, Kazutaka Tanabe, Kenji Takemoto, Kojiro Nakamura, Noriyuki Koyama, Masato Narita, Hiromitsu Nagata, Kojiro Taura, Shinji Uemoto. Effect of olprinone on liver microstructure in rat partial liver transplantation. The Journal of surgical research. 2013 Jul;183(1):391-6