Metabolic engineering of Escherichia coli for the production of 5-aminovalerate and glutarate as C5 platform chemicals.

Si Jae Park, Eun Young Kim, Won Noh, Hye Min Park, Young Hoon Oh, Seung Hwan Lee, Bong Keun Song, Jonggeon Jegal, Sang Yup Lee

Department of Environmental Engineering and Energy, Myongji University, San 38-2, Nam-dong, Cheoin-gu, Yongin-si, Gyeonggido 449-728, Republic of Korea.

Metabolic engineering 2013 Mar

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5-Aminovalerate (5AVA) is the precursor of valerolactam, a potential building block for producing nylon 5, and is a C5 platform chemical for synthesizing 5-hydroxyvalerate, glutarate, and 1,5-pentanediol. Escherichia coli was metabolically engineered for the production of 5-aminovalerate (5AVA) and glutarate. When the recombinant E. coli WL3110 strain expressing the Pseudomonas putidadavAB genes encoding delta-aminovaleramidase and lysine 2-monooxygenase, respectively, were cultured in a medium containing 20g/L of glucose and 10g/L of L-lysine, 3.6g/L of 5AVA was produced by converting 7g/L of L-lysine. When the davAB genes were introduced into recombinant E. coli strainXQ56allowing enhanced L-lysine synthesis, 0.27 and 0.5g/L of 5AVA were produced directly from glucose by batch and fed-batch cultures, respectively. Further conversion of 5AVA into glutarate could be demonstrated by expression of the P. putida gabTD genes encoding 5AVA aminotransferase and glutarate semialdehyde dehydrogenase. When recombinant E. coli WL3110 strain expressing the davAB and gabTD genes was cultured in a medium containing 20g/L glucose, 10g/L L-lysine and 10g/L α-ketoglutarate, 1.7g/L of glutarate was produced. Copyright © 2012 Elsevier Inc. All rights reserved.

Citation

Si Jae Park, Eun Young Kim, Won Noh, Hye Min Park, Young Hoon Oh, Seung Hwan Lee, Bong Keun Song, Jonggeon Jegal, Sang Yup Lee. Metabolic engineering of Escherichia coli for the production of 5-aminovalerate and glutarate as C5 platform chemicals. Metabolic engineering. 2013 Mar;16:42-7