Structural analysis of protein-protein interactions in type I polyketide synthases.

Wei Xu, Kangjian Qiao, Yi Tang

Critical reviews in biochemistry and molecular biology 2013 Mar-Apr

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Polyketide synthases (PKSs) are responsible for synthesizing a myriad of natural products with agricultural, medicinal relevance. The PKSs consist of multiple functional domains of which each can catalyze a specified chemical reaction leading to the synthesis of polyketides. Biochemical studies showed that protein-substrate and protein-protein interactions play crucial roles in these complex regio-/stereo-selective biochemical processes. Recent developments on X-ray crystallography and protein NMR techniques have allowed us to understand the biosynthetic mechanism of these enzymes from their structures. These structural studies have facilitated the elucidation of the sequence-function relationship of PKSs and will ultimately contribute to the prediction of product structure. This review will focus on the current knowledge of type I PKS structures and the protein-protein interactions in this system.

Citation

Wei Xu, Kangjian Qiao, Yi Tang. Structural analysis of protein-protein interactions in type I polyketide synthases. Critical reviews in biochemistry and molecular biology. 2013 Mar-Apr;48(2):98-122