BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing.

Here, we show that dBCAS2 (CG4980, human Breast Carcinoma Amplified Sequence 2 ortholog) is essential for the viability of Drosophila melanogaster. We find that ubiquitous or tissue-specific depletion of dBCAS2 leads to larval lethality, wing deformities, impaired splicing, and apoptosis. More importantly, overexpression of hBCAS2 rescues these defects. Furthermore, the C-terminal coiled-coil domain of hBCAS2 binds directly to CDC5L and recruits hPrp19/PLRG1 to form a core complex for splicing in mammalian cells and can partially restore wing damage induced by knocking down dBCAS2 in flies. In summary, Drosophila and human BCAS2 share a similar function in RNA splicing, which affects cell viability.

Citation

Po-Han Chen, Chia-I Lee, Yu-Tzu Weng, Woan-Yuh Tarn, Yeou-Ping Tsao, Ping-Chang Kuo, Pang-Hung Hsu, Chu-Wei Huang, Chiun-Sheng Huang, Hsiu-Hsiang Lee, June-Tai Wu, Show-Li Chen. BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing. RNA (New York, N.Y.). 2013 Feb;19(2):208-18

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PMID: 23249746

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