Facile determination of the absolute configurations of α-hydroxy acids by chiral derivatization coupled with liquid chromatography-mass spectrometry analysis.

Kyuho Moon, Chaemin Lim, Sanghee Kim, Dong-Chan Oh

Natural Products Research Institute, College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.

Journal of chromatography. A 2013 Jan 11

filter terms:

α-Hydroxy acids are commonly encountered in natural bioactive molecules. For convenient determination of the absolute configurations of α-hydroxy acids, a simple method, named the "O-Marfey method" was developed using a chiral derivatization strategy with (L-fluoro-2,4-dinitrophenyl-5)-L-alanine amide (L-FDAA). The liquid chromatography-mass spectrometry (LC-MS) analysis of the derivatives clearly determined the absolute configurations of various α-hydroxy acids based on their elution times and sequences. This new method is operationally simple with short reaction time and sensitive enough for application at a sub-milligram scale without any purification. This method also enables the simultaneous chirality analysis of α-hydroxy acids and α-amino acids. The absolute configuration of a natural depsipeptide bearing an α-hydroxy acid and α-amino acids was successfully determined by our O-Marfey application. Copyright © 2012 Elsevier B.V. All rights reserved.

Citation

Kyuho Moon, Chaemin Lim, Sanghee Kim, Dong-Chan Oh. Facile determination of the absolute configurations of α-hydroxy acids by chiral derivatization coupled with liquid chromatography-mass spectrometry analysis. Journal of chromatography. A. 2013 Jan 11;1272:141-4