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The separation and quantification of glycosaminoglycan (GAG) chains with different levels of sulfation from cells and media, and prepared through chemoenzymatic synthesis or metabolic engineering, pose a major challenge in glycomics analysis. A method for microscale separation and quantification of heparin, heparan sulfate, and heparosan from cells is reported. This separation relies on a mini strong anion exchange spin column eluted stepwise with various concentrations of sodium chloride. Disaccharide analysis by LC-MS was used to monitor the chemical structure of the various GAG chains that were recovered. Copyright © 2013 Elsevier Inc. All rights reserved.

Citation

Xue Zhao, Bo Yang, Kathryn Linkens, Payel Datta, Akihiro Onishi, Fuming Zhang, Robert J Linhardt. Microscale separation of heparosan, heparan sulfate, and heparin. Analytical biochemistry. 2013 Mar 15;434(2):215-7

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PMID: 23262074

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