Zijue Zhu, Wangjie Xu, Jingbo Dai, Xiaohui Chen, Xianglong Zhao, Peng Fang, Fang Yang, Maoping Tang, Zhaoxia Wang, Lianyun Wang, Duan Ma, Zhongdong Qaio
School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, PR China.
The international journal of biochemistry & cell biology 2013 MarSmoking is associated with a declining quality of semen. The aim of this study was to screen and investigate the differential expression of proteins extracted from the epididymis of mice exposed daily with cigarette smoke. Using MALDI-TOF-MS analysis, we found that the protein profile of the mouse epididymis was altered by cigarette smoking and identified 27 proteins from the most abundant and differentially expressed spots in the 2-DE gels of epididymal samples. These proteins were classified into groups according to their functions such as energy metabolism, reproduction and structural molecule activity. Through pathway analysis, these proteins were associated with the glutathione metabolism and protein processing in the endoplasmic reticulum. These results showed that the epididymis may experience oxidative stress following cigarette smoke exposure, which was confirmed using immunohistochemistry. We determine that cigarette smoking can induce oxidative stress in the mouse epididymis, which may cause protein profile altering, thereby impairing epididymis function, and leading to a decline in semen quality. Copyright © 2012 Elsevier Ltd. All rights reserved.
Zijue Zhu, Wangjie Xu, Jingbo Dai, Xiaohui Chen, Xianglong Zhao, Peng Fang, Fang Yang, Maoping Tang, Zhaoxia Wang, Lianyun Wang, Duan Ma, Zhongdong Qaio. The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis. The international journal of biochemistry & cell biology. 2013 Mar;45(3):571-82
PMID: 23262294
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