High-level production of thermotolerant β-xylosidase of Aspergillus sp. BCC125 in Pichia pastoris: characterization and its application in ethanol production.

Sriwan Wongwisansri, Peerada Promdonkoy, Panida Matetaviparee, Niran Roongsawang, Lily Eurwilaichitr, Sutipa Tanapongpipat

Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand. sriwan@biotec.or.th

Bioresource technology 2013 Mar

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A gene coding for thermotolerant β-xylosidase from Aspergillus sp. BCC125 was characterized. The recombinant enzyme was expressed in methylotrophic yeast Pichia pastoris KM71 and especially high yield of secreted enzyme was obtained. β-xylosidase possessed high enzyme efficiency (Kcat/Km=198.8mM(-1)s(-1)) toward pNP-β-D-xylopyranoside (pNPβX) with optimal temperature and pH for activity of 60°C and pH 4.0-5.0, respectively. The identified β-xylosidase showed clear synergism with previously identified xylanase for hydrolysis of xylan in vitro as well as simultaneous saccharification and fermentation process (SSF) in vivo with Pichia stipitis. Copyright © 2012 Elsevier Ltd. All rights reserved.

Citation

Sriwan Wongwisansri, Peerada Promdonkoy, Panida Matetaviparee, Niran Roongsawang, Lily Eurwilaichitr, Sutipa Tanapongpipat. High-level production of thermotolerant β-xylosidase of Aspergillus sp. BCC125 in Pichia pastoris: characterization and its application in ethanol production. Bioresource technology. 2013 Mar;132:410-3