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In this report, we present a novel approach for the elucidation of the physicochemical properties of lipid membranes by isothermal titration calorimetry (ITC) to quantify the heat absorbed during the solubilization of vesicles into TritonX-100 micelles. By using large and small unilamellar vesicles for comparison, this method provides calorimetric data on the gel-to-liquid-crystalline phase transition and its curvature effects and, in particular, the enthalpy change upon membrane deformation from a planar to a curved shape, which cannot be obtained by the conventional approach using differential scanning calorimetry. The results showed quantitatively that the increase in membrane curvature increases the enthalpy of 1,2-dimyristoyl-sn-glycero-3-phosphocholine membranes both below and above the phase-transition temperature, and that the effect is more significant for the former condition. The calorimetric data obtained are further discussed in relation to the elastic bending energy of the membranes and membrane-peptide interaction.


Hirokazu Yokoyama, Keisuke Ikeda, Masaki Wakabayashi, Yasushi Ishihama, Minoru Nakano. Effects of lipid membrane curvature on lipid packing state evaluated by isothermal titration calorimetry. Langmuir : the ACS journal of surfaces and colloids. 2013 Jan 22;29(3):857-60

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PMID: 23270307

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