Suozhu Shi, Qingli Cheng, Ping Zhang, Nan Wang, Ying Zheng, Xue-Yuan Bai, Xiangmei Chen
Dept of Nephrology, State Key Lab of Kidney Diseases, Chinese PLA General Hospital, Beijing, China.
American journal of clinical pathology 2013 JanImmunofluorescence of frozen tissue sections (IF-F) is a classic technique for renal immunopathologic examination. However, it has certain disadvantages, such as diffuse antigen distribution and few or even no glomeruli in the section. We developed a new technique of immunofluorescence staining using dual microwave retrieval in paraffin-embedded renal tissue sections (IF-DMP) and compared IF-DMP with IF-F in 406 renal biopsy samples. IF-DMP detected significantly more glomeruli than did IF-F (P< .001). There was no significant difference for the specificity and sensitivity in the detection of immunoglobulins, complements, κ, and λ between IF-F and IF-DMP. Concordant observations were 98% for all immunofluorescence, complements, κ, and λ staining and 100% for immunoglobulin staining. Both techniques were completely accurate in confirming diagnoses of various glomerular diseases. IF-DMP provided clearer images of tissue structure and more precise localization of antigens, and it is a suitable alternative for traditional IF-F in clinical renal immunopathologic diagnosis.
Suozhu Shi, Qingli Cheng, Ping Zhang, Nan Wang, Ying Zheng, Xue-Yuan Bai, Xiangmei Chen. Immunofluorescence with dual microwave retrieval of paraffin-embedded sections in the assessment of human renal biopsy specimens. American journal of clinical pathology. 2013 Jan;139(1):71-8
PMID: 23270901
View Full Text