Small hepatitis B surface antigen interacts with and modulates enoyl-coenzyme A hydratase expression in hepatoma cells.

Xianqiong Gong, Yueyong Zhu, Jing Dong, Jing Chen, Jia You, Qi Zheng, Zilan Rao, Qianguo Mao, Jiaji Jiang

Center of Liver Diseases, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian, China.

Archives of virology 2013 May

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Enoyl-coenzyme A hydratase (ECHS1) is a key enzyme in the metabolism of fatty acids in mitochondria. We previously reported that hepatitis B surface antigen (HBsAg) interacted with ECHS1 in a yeast two-hybrid system. In the current study, we further examined their interaction by using GST pull-down and co-immunoprecipitation assays. The results confirmed that small hepatitis B surface antigen (SHBs) interacted with ECHS1. Furthermore, confocal imaging showed that SHBs and ECHS1 co-localized in HepG2 cells. To clarify the biological function of the interaction, human hepatoma cell lines that transiently and stably expressed SHBs were generated. The expression of SHBs led to a significant decrease in ECHS1 protein levels. ECHS1 protein levels were reduced to 48.44 ± 7.12 % in Huh7 cells transiently expressing SHBs, and to 54.97 ± 3.54 % in HepG2 cells stably expressing SHBs. In conclusion, our findings suggest that SHBs interacts with ECHS1 and regulates ECHS1 protein levels in hepatoma cells.

Citation

Xianqiong Gong, Yueyong Zhu, Jing Dong, Jing Chen, Jia You, Qi Zheng, Zilan Rao, Qianguo Mao, Jiaji Jiang. Small hepatitis B surface antigen interacts with and modulates enoyl-coenzyme A hydratase expression in hepatoma cells. Archives of virology. 2013 May;158(5):1065-70