Glucose-6-phosphate dehydrogenase (G6PD) gene mutations detection by improved high-resolution DNA melting assay.

Meichen Pan, Min Lin, Lin Yang, Jiaoren Wu, Xiaofen Zhan, Ying Zhao, Yingfang Wen, Guirong Liu, Liye Yang, Yingmu Cai

Molecular biology reports 2013 Apr

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Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a common inherited disorder worldwide including southern China. G6PD gene mutations cause deficiency of the enzyme and a large spectrum of diseases. High-resolution DNA melting (HRM) assay was recently introduced as a rapid, inexpensive and effective method for genotyping. But there was a shortcoming of this method that hemizygous and homozygous genotypes were not easily distinguished from wild-types. Here we used improved HRM method for a small-scale screening of G6PD-deficient variants among people of Meizhou region. Then all amplicons were ascertained by direct DNA sequencing. These results indicated that HRM method was a major technical advance for G6PD mutations screening.

Citation

Meichen Pan, Min Lin, Lin Yang, Jiaoren Wu, Xiaofen Zhan, Ying Zhao, Yingfang Wen, Guirong Liu, Liye Yang, Yingmu Cai. Glucose-6-phosphate dehydrogenase (G6PD) gene mutations detection by improved high-resolution DNA melting assay. Molecular biology reports. 2013 Apr;40(4):3073-82