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The main aim of this study was to evaluate the toxicity of different poultry manure landfill leachates, using a well-known toxicity test system (MS3). The bioassay was made using a battery of toxicity tests including acute toxicity with crustacean (Daphnia magna), algae (Chlorella vulgaris) and the in vitro toxicity test with the fish cell line RTG-2. On D. magna was high mortality for zero time and almost 100 % and 70 %-80 % mortality for sawdust and straw poultry manure respectively. No effects on C. vulgaris, was observed after the leachate exposure. None of the parameters considered: protein, EROD activity, β-gal activity and neutral red, showed differences between control test and the leachate collected from exposure to poultry manure.


M Delgado, R Miralles de Imperial, F Alonso, C Rodríguez, J V Martín. Ecotoxicity bioassays on leachates from poultry manure. Bulletin of environmental contamination and toxicology. 2013 Apr;90(4):401-4

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PMID: 23283533

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