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An up-to-date overview of thin-layer chromatography (TLC) techniques for chiral separations of various significant and/or recent examples of enantioresolutions is reported. Furthermore, examples for chiral separations obtained on achiral commercially available C18 TLC plates are described in detail. These include the enantioseparation of methylthiohydantoin-phenylalanine and methylthiohydantoin-tyrosine using hydroxyethyl-β-cyclodextrin as mobile phase additive and the separation of the enantiomers of warfarin and p-chlorowarfarin using bovine serum albumin as mobile phase additive.


Massimo Del Bubba, Leonardo Checchini, Alessandra Cincinelli, Luciano Lepri. Enantioseparations by thin-layer chromatography. Methods in molecular biology (Clifton, N.J.). 2013;970:29-43

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PMID: 23283769

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