Evaluation of breast cancer resistance protein function in hepatobiliary and renal excretion using PET with 11C-SC-62807.

Tadayuki Takashima, Chunyong Wu, Misato Takashima-Hirano, Yumiko Katayama, Yasuhiro Wada, Masaaki Suzuki, Hiroyuki Kusuhara, Yuichi Sugiyama, Yasuyoshi Watanabe

Molecular Probe Dynamics Laboratory, RIKEN Center for Molecular Imaging Science, Kobe, Japan. ttakashima@riken.jp

Journal of nuclear medicine : official publication, Society of Nuclear Medicine 2013 Feb

filter terms:

A quantitative PET imaging method was used to assess the in vivo kinetics of hepatobiliary and renal excretion of the breast cancer resistance protein (Bcrp) substrate (11)C-SC-62807 in mice. Serial abdominal PET scans were collected in wild-type and Bcrp knockout (Bcrp(-/-)) mice after intravenous injection of (11)C-SC-62807. Venous blood samples and PET images were obtained at frequent intervals up to 30 min after radiotracer administration. Dynamic PET data were analyzed to determine the canalicular and brush-border efflux clearances in the liver and kidney (CL(int,bile,liver) and CL(int,urine,kidney), respectively). SC-62807 is an in vitro substrate of mouse Bcrp and human BCRP. Radioactivity associated with (11)C-SC-62807 was predominantly found in the blood, liver, bile, and urine 30 min after administration. Both biliary and urinary excretion of radioactivity was markedly lower in Bcrp(-/-) mice than in wild-type mice, suggesting greater systemic exposure in Bcrp(-/-) mice. Both the CL(int,bile,liver) and the CL(int,urine,kidney) were significantly lower in Bcrp(-/-) mice (74% ± 10% and 99% ± 1% lower than controls, respectively). We also found that (11)C-SC-62807 is a substrate of the organic anion-transporting polypeptides OATP1B1 and OATP1B3 in vitro. The present study demonstrated that Bcrp plays a significant role in the efflux of (11)C-SC-62807 in mouse liver and kidney. We also demonstrated the feasibility of PET using (11)C-SC-62807 to study the activity of BCRP in humans.

Citation

Tadayuki Takashima, Chunyong Wu, Misato Takashima-Hirano, Yumiko Katayama, Yasuhiro Wada, Masaaki Suzuki, Hiroyuki Kusuhara, Yuichi Sugiyama, Yasuyoshi Watanabe. Evaluation of breast cancer resistance protein function in hepatobiliary and renal excretion using PET with 11C-SC-62807. Journal of nuclear medicine : official publication, Society of Nuclear Medicine. 2013 Feb;54(2):267-76