Interaction of carboxylated single-walled carbon nanotubes with bovine serum albumin.

Lili Li, Rui Lin, Hua He, Li Jiang, Mengmeng Gao

China Pharmaceutical University, Nanjing 210009, China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2013 Mar 15

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Carboxylated single-walled carbon nanotubes (c-SWNTs) were synthesized prosperously in order to improve dispersion of raw carbon nanotubes. Then, bovine serum albumin (BSA) was used as the template protein to study the biocompatibility of c-SWNTs by UV-Vis, fluorescence and circular dichroism (CD) spectroscopic methods at the molecular level. Results from fluorescence spectrum showed obvious decreases in fluorescence intensity of BSA induced by c-SWNTs, indicating the occurrence of interaction between BSA and c-SWNTs. Static quenching effect of c-SWNTs was verified by linear Stern-Volmer plots and K(SV) values. Thermodynamic parameters at different temperatures demonstrated that the interaction between c-SWNTs and BSA was mainly favored by hydrophobic force. In addition, Na(+) interfered with the quenching effect of c-SWNTs, which revealed that electrostatic force played a role in binding roles of BSA to c-SWNTs simultaneously. The results of UV and synchronous fluorescence spectrum validated that hydrophobicity of amino acid residues expressly increased with the addition of c-SWNTs. The content of α-helix structure in BSA decreased by 14.06% with c-SWNTs viewed from CD spectrum. Effect of SWNTs on the conformation of BSA could be controlled by the surface chemistry of SWNTs. Copyright © 2012 Elsevier B.V. All rights reserved.

Citation

Lili Li, Rui Lin, Hua He, Li Jiang, Mengmeng Gao. Interaction of carboxylated single-walled carbon nanotubes with bovine serum albumin. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 2013 Mar 15;105:45-51