Yumei Yan, Jianbin Huang, Fangbao Ding, Ju Mei, Jiaquan Zhu, Hao Liu, Kun Sun
Department of Cardiothoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, PR China.
International journal of molecular medicine 2013 MarMyocardial stunning, which is closely related to myocardial edema, is a severe complication that may occur following cardiac surgery. In this study, we examined the expression of aquaporin 1 (AQP1) and Connexin 43 (Cx43) following cardiopulmonary bypass (CPB) surgery in goats. We assessed myocardial muscle tissue water content according to changes in dry-wet weight. Our results showed that AQP1 expression and myocardial muscle tissue water content increased significantly 6 h after CPB surgery, reaching peak levels 48 h after surgery; additionally, the protein expression of Cx43 was inversely correlated with AQP1 expression. Overexpression of AQP1 during CPB surgery enhanced the degree of myocardial edema, whereas the addition of water channel protein inhibitor Hg2+ in cold crystalloid cardioplegia and knockdown of AQP1 during surgery weakened the degree of myocardial edema. These findings revealed that the severity of myocardial edema after CPB surgery is correlated with AQP1 protein expression levels, suggesting the important role played by AQP1 protein in the regulation of Cx43 in the pathological progression of myocardial edema.
Yumei Yan, Jianbin Huang, Fangbao Ding, Ju Mei, Jiaquan Zhu, Hao Liu, Kun Sun. Aquaporin 1 plays an important role in myocardial edema caused by cardiopulmonary bypass surgery in goat. International journal of molecular medicine. 2013 Mar;31(3):637-43
PMID: 23292298
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