Johannes Hradsky, Marina Mikhaylova, Anna Karpova, Michael R Kreutz, Werner Zuschratter
Research Group, Neuroplasticity, Leibniz Institute for Neurobiology (LIN), Magdeburg, Germany.
Methods in molecular biology (Clifton, N.J.) 2013Calcium (Ca(2+)) signaling in neurons is mediated by plethora of calcium binding proteins with many of them belonging to the Calmodulin family of calcium sensors. Many studies have shown that the subcellular localization of neuronal EF-hand Ca(2+)-sensors is crucial for their cellular function. To overcome the resolution limit of classical fluorescence and confocal microscopy various imaging techniques have been developed recently that improve the resolution by an order of magnitude in all dimensions. This new microscope techniques make co-localization studies of Ca(2+)-binding proteins more reliable and help to get insights into the macromolecular organization of intracellular structures and signaling pathways beyond the diffraction limit of visible light.
Johannes Hradsky, Marina Mikhaylova, Anna Karpova, Michael R Kreutz, Werner Zuschratter. Super-resolution microscopy of the neuronal calcium-binding proteins Calneuron-1 and Caldendrin. Methods in molecular biology (Clifton, N.J.). 2013;963:147-69
PMID: 23296610
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