Correlation Engine 2.0
Clear Search sequence regions

Protein carbonylation is the most commonly used measure of oxidative modification of proteins. It is most often measured spectrophotometrically or immunochemically by derivatizing proteins with the classical carbonyl reagent 2,4 dinitrophenylhydrazine (DNPH). We present protocols for the derivatization and quantification of protein carbonylation with these two methods, including a newly described dot blot with greatly increased sensitivity.


Nancy B Wehr, Rodney L Levine. Quantification of protein carbonylation. Methods in molecular biology (Clifton, N.J.). 2013;965:265-81

Expand section icon Mesh Tags

Expand section icon Substances

PMID: 23296665

View Full Text