Detection and cellular localization of phospho-STAT2 in the central nervous system by immunohistochemical staining.

Reza Khorooshi, Trevor Owens

Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark.

Methods in molecular biology (Clifton, N.J.) 2013

filter terms:

Phosphorylation of signal transducers and activators of transcription (STATs) indicates their involvement in active signaling. Here we describe immunohistochemical staining procedures for detection and identification of the cellular localization of phospho-STAT2 in the central nervous system (CNS) of mice. The procedures include single and dual/triple labeling immunostaining of phospho-STAT2 in the hippocampus of mice following entorhinal cortex lesion.

Citation

Reza Khorooshi, Trevor Owens. Detection and cellular localization of phospho-STAT2 in the central nervous system by immunohistochemical staining. Methods in molecular biology (Clifton, N.J.). 2013;967:179-88