Application of chip-based flow cytometry for amphotericin B and fluconazole susceptibility testing on Candida strains.

Orsolya Bouquet, Béla Kocsis, Ferenc Kilár, Ildikó Kustos

Department of Analytical and Environmental Chemistry, Faculty of Sciences, University of Pécs, Pécs, Hungary. bouquetorsi@gmail.com

Methods in molecular biology (Clifton, N.J.) 2013

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Chip-based flow cytometry is a rather new method that offers an easy, fast opportunity for examination of yeasts, such as Candida cells. In our study cell-chip technology was tested with ATCC Candida strains to determine their viability and susceptibility against antifungal agents, amphotericin B and fluconazole. We found this technology to be suitable for the detection of Candida cells, for the differentiation between dead and living cells, and for the determination of amphotericin B and fluconazole susceptibility of different Candida strains (Bouquet et al., Mycoses 55:e90-e96, 2012).

Citation

Orsolya Bouquet, Béla Kocsis, Ferenc Kilár, Ildikó Kustos. Application of chip-based flow cytometry for amphotericin B and fluconazole susceptibility testing on Candida strains. Methods in molecular biology (Clifton, N.J.). 2013;968:149-54