Enrichment of methylated DNA by methyl-CpG immunoprecipitation.

Miriam Sonnet, Constance Baer, Michael Rehli, Dieter Weichenhan, Christoph Plass

Department of Epigenomics and Cancer Risk Factors, German Cancer Research Center (DKFZ), Heidelberg, Germany.

Methods in molecular biology (Clifton, N.J.) 2013

filter terms:

Normal DNA methylation is an epigenetic modification required for proper development. Aberrant DNA methylation, in contrast, is frequently observed in many different malignancies including leukemias and lymphomas. Global DNA methylation profiling addresses the methylated sequences (methylome) of patient genomes to identify disease-specific methylation patterns. Workload in methylome analyses can be considerably reduced by methylome enrichment using proteins or antibodies with high affinity to methylated DNA. Methyl-CpG Immunoprecipitation (MCIp) employs an immobilized recombinant human methyl-CpG binding domain protein 2, MBD2, which binds methylated CpGs in double-stranded DNA. Elution with increasing salt concentrations allows the fractionated enrichment of different degrees of methylation.

Citation

Miriam Sonnet, Constance Baer, Michael Rehli, Dieter Weichenhan, Christoph Plass. Enrichment of methylated DNA by methyl-CpG immunoprecipitation. Methods in molecular biology (Clifton, N.J.). 2013;971:201-12