Metallofullerene nanoparticles promote osteogenic differentiation of bone marrow stromal cells through BMP signaling pathway.

Kangning Yang, Weipeng Cao, Xiaohong Hao, Xue Xue, Jing Zhao, Juan Liu, Yuliang Zhao, Jie Meng, Baoyun Sun, Jinchao Zhang, Xing-Jie Liang

Laboratory of Nanomedicine and Nanosafety, Division of Nanomedicine and Nanobiology, National Center for Nanoscience and Technology of China, Chinese Academy of Sciences, No. 11, First North Road, Zhongguancun, Beijing, 100190, China.

Nanoscale 2013 Feb 7

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Although endohedral metallofullerenol [Gd@C(82)(OH)(22)](n) nanoparticles have anti-tumor efficiency and mostly deposit in the bones of mice, how these nanoparticles act in bone marrow stromal cells (MSCs) remains largely unknown. Herein, we observed that [Gd@C(82)(OH)(22)](n) nanoparticles facilitated the differentiation of MSCs toward osteoblasts, as evidenced by the enhancement of alkaline phosphatase (ALP) activity and mineralized nodule formation upon [Gd@C(82)(OH)(22)](n) nanoparticle treatment. Mechanistically, the effect of [Gd@C(82)(OH)(22)](n) nanoparticles on ALP activity was inhibited by the addition of noggin as an inhibitor of the BMP signaling pathway. Moreover, the in vivo results of the ovariectomized rats further indicated that [Gd@C(82)(OH)(22)](n) nanoparticles effectively improved bone density and prevented osteoporosis.

Citation

Kangning Yang, Weipeng Cao, Xiaohong Hao, Xue Xue, Jing Zhao, Juan Liu, Yuliang Zhao, Jie Meng, Baoyun Sun, Jinchao Zhang, Xing-Jie Liang. Metallofullerene nanoparticles promote osteogenic differentiation of bone marrow stromal cells through BMP signaling pathway. Nanoscale. 2013 Feb 7;5(3):1205-12