The Vibrio harveyi bioassay used routinely to detect AI-2 quorum sensing inhibition is confounded by inconsistent normalization across marine matrices.

Walter M Blair, Gregory J Doucette

Graduate Program in Marine Biology, College of Charleston, Charleston, SC 29412, USA.

Journal of microbiological methods 2013 Mar

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The Vibrio harveyi autoinducer-2 (AI-2) bioassay is used routinely to screen for inhibition of the AI-2 quorum sensing system. The present study utilizes three well-described bacterial strains to demonstrate that inconsistent normalization across matrices undermines the assay's use in screening marine samples for AI-2 inhibition. Published by Elsevier B.V.

Citation

Walter M Blair, Gregory J Doucette. The Vibrio harveyi bioassay used routinely to detect AI-2 quorum sensing inhibition is confounded by inconsistent normalization across marine matrices. Journal of microbiological methods. 2013 Mar;92(3):250-2