A serine-to-asparagine mutation at position 314 of H5N1 avian influenza virus NP is a temperature-sensitive mutation that interferes with nuclear localization of NP.

Nattamon Siboonnan, Wittawat Wiriyarat, Chompunuch Boonarkart, Warunya Chakritbudsabong, Anan Jongkaewwattana, Pilaipan Puthavathana, Prasert Auewarakul, Ornpreya Suptawiwat

Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Prannok Road, Bangkoknoi, Bangkok 10700, Thailand.

Archives of virology 2013 Jun

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We have generated a temperature-sensitive (ts) mutant from a human isolate of the H5N1 avian influenza virus by classical adaptation in cell culture. After 20 passages at low temperature, the virus showed a ts phenotype. The ts mutant also showed an attenuated phenotype after nasal inoculation in mice. Using reverse genetics, we generated reassortants carrying individual genomic segments of the wild-type and mutant viruses in an A/Puerto Rico/8/34 background, and found that the nucleoprotein (NP) gene could confer the ts phenotype. This mutant NP contains a serine-to-asparagine mutation at position 314 (S314N). The mutant NP protein showed a defect in nuclear localization at high temperature in mammalian cells.

Citation

Nattamon Siboonnan, Wittawat Wiriyarat, Chompunuch Boonarkart, Warunya Chakritbudsabong, Anan Jongkaewwattana, Pilaipan Puthavathana, Prasert Auewarakul, Ornpreya Suptawiwat. A serine-to-asparagine mutation at position 314 of H5N1 avian influenza virus NP is a temperature-sensitive mutation that interferes with nuclear localization of NP. Archives of virology. 2013 Jun;158(6):1151-7