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The fabrication and implementation of aligned electrospun polyacrylonitrile (PAN) nanofibers as a stationary phase for ultra-thin layer chromatography (UTLC) is described. The aligned electrospun UTLC plates (AE-UTLC) were characterized to give an optimized electrospun mat consisting of high nanofiber alignment and a mat thickness of ~25 μm. The AE-UTLC devices were used to separate a mixture of β-blockers and steroidal compounds to illustrate the properties of AE-UTLC. The AE-UTLC plates provided shorter analysis time (~2-2.5 times faster) with improved reproducibility (as high as 2 times) as well as an improvement in efficiency (up to100 times greater) relative to non-aligned electrospun-UTLC (E-UTLC) devices. Copyright © 2012 Elsevier B.V. All rights reserved.


Michael C Beilke, Joseph W Zewe, Jonathan E Clark, Susan V Olesik. Aligned electrospun nanofibers for ultra-thin layer chromatography. Analytica chimica acta. 2013 Jan 25;761:201-8

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PMID: 23312332

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