Novel cellulase recycling method using a combination of Clostridium thermocellum cellulosomes and Thermoanaerobacter brockii β-glucosidase.

Rattiya Waeonukul, Akihiko Kosugi, Panida Prawitwong, Lan Deng, Chakrit Tachaapaikoon, Patthra Pason, Khanok Ratanakhanokchai, Masayoshi Saito, Yutaka Mori

Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 303-8686, Japan.

Bioresource technology 2013 Feb

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This report describes a novel recycling method utilizing a combination of Clostridium thermocellum cellulosomes and Thermoanaerobacter brockii β-glucosidase (CglT). To recover cellulosomes and CglT through re-binding to additional cellulose, a chimeric CBM3-CglT was created by fusing carbohydrate binding module (CBM3) from the scaffolding protein CipA into the N-terminal region of CglT. When a recycling test using cellulosomes and CBM3-CglT was performed on microcrystalline cellulose, the process was capable of 4 rounds of recycling (1%w/vcellulose/round). Although irreversible absorption of cellulosomes and CBM3-CglT into the residues was observed when ammonia-pretreated rice straw and delignified rice straw was used as substrates, a maximum of 2 and 4 recycling rounds (1%w/vglucan/round) were achieved, respectively, consistent with a 70% saccharification rate. This novel recycling method using cellulosomes and CBM3-CglT has great potential as an effective lignocellulose degradation system. Copyright © 2012 Elsevier Ltd. All rights reserved.

Citation

Rattiya Waeonukul, Akihiko Kosugi, Panida Prawitwong, Lan Deng, Chakrit Tachaapaikoon, Patthra Pason, Khanok Ratanakhanokchai, Masayoshi Saito, Yutaka Mori. Novel cellulase recycling method using a combination of Clostridium thermocellum cellulosomes and Thermoanaerobacter brockii β-glucosidase. Bioresource technology. 2013 Feb;130:424-30