rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells.

Yasuko Onuma, Hiroaki Tateno, Jun Hirabayashi, Yuzuru Ito, Makoto Asashima

Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan.

Biochemical and biophysical research communications 2013 Feb 15

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Cell surface biomarkers have been applied to discriminate pluripotent human embr yonic stem cells and induced pluripotent stem cells from differentiated cells. Here, we demonstrate that a recombinant lectin probe, rBC2LCN, a new tool for fluorescence-based imaging and flow cytometry analysis of pluripotent stem cells, is an alternative to conventional pluripotent maker antibodies. Live or fixed colonies of both human embryonic stem cells and induced pluripotent stem cells were visualized in culture medium containing fluorescent dye-labeled rBC2LCN. Fluorescent dye-labeled rBC2LCN was also successfully used to separate live pluripotent stem cells from a mixed cell population by flow cytometry. Copyright © 2013 Elsevier Inc. All rights reserved.

Citation

Yasuko Onuma, Hiroaki Tateno, Jun Hirabayashi, Yuzuru Ito, Makoto Asashima. rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells. Biochemical and biophysical research communications. 2013 Feb 15;431(3):524-9