Lin Zhang, Lan Du, Wen-Wen Lv, Qing-Chun Zhao, Wei Hua, Ye An, Tao Guo, Li-Jun Wu
Department of Pharmacy, General Hospital of Shenyang Military Area Command, Shenyang 110840, China.
Journal of Asian natural products research 2013Four new metabolites M-1 [1,2,18,19-tetradehydro-4-demethyl-3,17-epoxy-7,20(2H,19H)-cyclovobasan], M-2 [1,2,4,21,18,19-hexadehydro-4-demethyl-3,17-epoxy-7,20(2H,19H)-cyclovobasan], M-3 [1,2,18,19-tetradehydro-4-demethyl-4-formaldehyde-3,17-epoxy-7,20(2H,19H)-cyclovobasan], and M-4 [1,2,4,21,18,19-hexadehydro-4-demethyl-4-oxy-3,17-epoxy-7,20(2H,19H)-cyclovobasan] were isolated from the chloroform extract of koumine incubated with phenobarbital-treated rat liver microsomes. The structures of M-1, M-2, M-3, and M-4 were elucidated by spectroscopic methods including ESI-TOF-MS, 1D, and 2D NMR experiments. The metabolic pathway of koumine was proposed. The cytotoxic activities between koumine and its metabolites were also compared in the A549 cell line.
Lin Zhang, Lan Du, Wen-Wen Lv, Qing-Chun Zhao, Wei Hua, Ye An, Tao Guo, Li-Jun Wu. Four new koumine metabolites in rat liver microsomes. Journal of Asian natural products research. 2013;15(1):46-52
PMID: 23323600
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