Lamin A/C is expressed in pluripotent mouse embryonic stem cells.

The pluripotent nature of embryonic stem cells (ESC) is associated with a dynamic open chromatin state and an irregular nuclear shape. It has been postulated that the absence of Lamin A/C contributes to these features. However, we show that mouse ESCs express low, yet readily detectable, amounts of Lamin A/C at both the RNA and protein levels. Full-length transcripts of both isoforms were readily detected by q-PCR and deep RNA sequencing. Additionally, protein expression was validated in multiple primary and established ESC lines by immunoblotting using several independent antibodies. Immunofluorescence labeling showed localization of Lamin A/C at the nuclear periphery of all Oct4/Nanog double-positive ESC lines examined, as well as in the inner cell mass of blastocysts. Our results demonstrate ESCs do express low levels of Lamin A/C, thus models linking pluripotency and nuclear dynamics with the absence of Lamin A/C need to be revisited.

Citation

Melanie A Eckersley-Maslin, Jan H Bergmann, Zsolt Lazar, David L Spector. Lamin A/C is expressed in pluripotent mouse embryonic stem cells. Nucleus (Austin, Tex.). 2013 Jan-Feb;4(1):53-60

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PMID: 23324457

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