Michael R Fischer, Dominik John, Kordula Kautz-Neu, Anja I Schermann, Kirsten Schwonberg, Esther von Stebut
Department of Dermatology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.
Methods in molecular biology (Clifton, N.J.) 2013Using cutaneous leishmaniasis of mice, the existence of so-called T helper (Th) cells type 1 and type 2 had been identified more than 20 years ago. Nowadays, it is well accepted that additional T cell populations as well as B cell-mediated immunity is required for immunity against Leishmania major. Finally, using inbred mouse strains, the relevance of genetical factors that influence anti-pathogen immunity as well as elements of the skin-immune system have been identified. This protocol describes a model for murine experimental leishmaniasis that tries to mimic natural parasite transmission by several means: (1) utilization of only infectious-stage parasites that are found in sand fly saliva, (2) intradermal inoculation, and (3) infection with only 1,000 parasites similar to the numbers inoculated by an infected sand fly.
Michael R Fischer, Dominik John, Kordula Kautz-Neu, Anja I Schermann, Kirsten Schwonberg, Esther von Stebut. Animal model for cutaneous leishmaniasis. Methods in molecular biology (Clifton, N.J.). 2013;961:389-402
PMID: 23325659
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