Correlation Engine 2.0
Clear Search sequence regions

The major histocompatibility complex (MHC) class I restricted pathway of antigen processing allows the presentation of intracellular antigens to cytotoxic T lymphocytes. The proteasome is the main protease in the cytoplasm and the nucleus, which is responsible for the generation of most peptide ligands of MHC-I molecules. Peptides produced by the proteasome can be further trimmed or destroyed by numerous cytosolic or endoplasmic reticulum (ER) lumenal proteases. Small molecule inhibitors are useful tools for probing the role of proteases in MHC class I antigen processing. Here, we describe different methods to test the impact of protease inhibitors in antigen presentation assays.


Michael Basler, Marcus Groettrup. Using protease inhibitors in antigen presentation assays. Methods in molecular biology (Clifton, N.J.). 2013;960:31-9

Expand section icon Mesh Tags

Expand section icon Substances

PMID: 23329476

View Full Text