Assembly of matched alpha/beta subunits to HLA class II peptide receptors.

Sebastian Temme, Nadine Kämper, Norbert Koch

Institute of Molecular Cardiology, University of Düsseldorf, Düsseldorf, Germany. sebastian.temme@uni-duesseldorf.de

Methods in molecular biology (Clifton, N.J.) 2013

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Human antigen presenting cells express three human leukocyte antigen (HLA) class II isotypes (DR, DP, and DQ), which are composed of polymorphic α and β subunits. The combination of polymorphic α- and β-chains results in cis (encoded on the same chromosome) or trans (encoded on different chromosomes) combinations. Since some of the α-β combinations may yield mismatched non-functional α-β heterodimers, it is not entirely clear which type of HLA class II peptide receptors are found on the cell surface of antigen presenting cells. We have developed a combination of biochemical techniques for inspection of the assembly and intracellular transport of isotype matched and mismatched class II heterodimers.

Citation

Sebastian Temme, Nadine Kämper, Norbert Koch. Assembly of matched alpha/beta subunits to HLA class II peptide receptors. Methods in molecular biology (Clifton, N.J.). 2013;960:433-45